Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain

Gene Ther. 2003 Nov;10(23):1933-40. doi: 10.1038/sj.gt.3302094.

Abstract

HIV-derived lentiviral vectors are efficient vehicula to deliver genes into the brain and hold great promise for future gene therapy of neurodegenerative disorders. However, administration of the current vector preparations in mouse brain was found to induce a systemic immune response to vector proteins and a modest inflammation in the brain. Moreover, serum antibodies from vector-treated animals were capable of partially neutralizing lentiviral vector-mediated transduction in cell culture. To avoid this unexpected immune reaction, we have optimized new vector production and purification protocols. Purification by sucrose gradient ultracentrifugation abolished the immune response, but vector titers also decreased substantially. Lentiviral vector production in the absence of serum in the cell culture medium equally reduced immunogenicity without affecting transduction efficiency. These results have important implications for future clinical use of lentiviral vectors, and for the use of lentiviral vectors to create animal models for neurodegenerative diseases that have an important neuroinflammatory component.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / blood
  • Brain / immunology*
  • Brain / metabolism
  • Culture Media, Serum-Free
  • Female
  • Gene Expression
  • Genetic Therapy / adverse effects*
  • Genetic Therapy / methods
  • Genetic Vectors / administration & dosage
  • Genetic Vectors / immunology*
  • Green Fluorescent Proteins
  • HIV-1 / genetics*
  • HIV-1 / immunology
  • Injections, Intraventricular
  • Luminescent Proteins / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Transduction, Genetic / methods*
  • Virus Cultivation

Substances

  • Antibodies
  • Culture Media, Serum-Free
  • Luminescent Proteins
  • Green Fluorescent Proteins