Abstract
The stepwise chromatographic behaviour on DEAE-Sepharose of rat Fe65, a neuronal protein, was tested, using as eluants KCl, CaCl2, and MgCl2. Assays by western blot showed that Fe65 was eluted by CaCl2, at a ionic strength 20% lower than that of MgCl2 or KCl. Interestingly, in the case of a truncated Fe65, lacking a glutamic acid rich region at the N-terminus, the ionic strengths of the various eluants were almost identical. These results suggested a possible inhibitory role of calcium ions in the binding of the protein to DEAE and a specific affinity of these ions for long acidic stretches.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Blotting, Western
-
Calcium Chloride / chemistry
-
Calcium Chloride / metabolism
-
Chromatography, Liquid / instrumentation
-
Chromatography, Liquid / methods
-
Magnesium Chloride / chemistry
-
Magnesium Chloride / metabolism
-
Nerve Tissue Proteins / chemistry*
-
Nerve Tissue Proteins / metabolism
-
Nuclear Proteins / chemistry*
-
Nuclear Proteins / metabolism
-
Osmolar Concentration
-
Peptide Fragments / chemistry
-
Peptide Fragments / metabolism
-
Protein Structure, Tertiary
-
Rats
-
Sepharose / analogs & derivatives*
-
Sepharose / metabolism*
Substances
-
Apbb1 protein, rat
-
Nerve Tissue Proteins
-
Nuclear Proteins
-
Peptide Fragments
-
Magnesium Chloride
-
Sepharose
-
Calcium Chloride