Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

Biotechnol Lett. 2003 Aug;25(16):1325-8. doi: 10.1023/a:1024951322955.

Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

Publication types

  • Evaluation Study

MeSH terms

  • Biotin / chemistry*
  • Calorimetry / methods*
  • Coated Materials, Biocompatible / chemistry*
  • Equipment Failure Analysis / methods
  • Macromolecular Substances
  • Polymerase Chain Reaction / instrumentation*
  • Polymerase Chain Reaction / methods*
  • Polypropylenes*
  • Protein Binding
  • Protein Interaction Mapping / methods
  • Spectrometry, Fluorescence / methods*
  • Streptavidin / chemistry*

Substances

  • Coated Materials, Biocompatible
  • Macromolecular Substances
  • Polypropylenes
  • Biotin
  • Streptavidin