Conjugate DNAzymes

Nucleic Acids Res Suppl. 2003:(3):177-8. doi: 10.1093/nass/3.1.177.

Abstract

Conjugate DNAzymes were synthesized by solid phase fragment condensation and their biological properties were characterized. They have increased affinity to target RNA, enhanced stability against DNase 1 digestion and comparable or higher RNA cleaving activity compared with native and also phosphorothioate DNA zymes. It was also demonstrated that conjugate DNAzymes could inhibit BCR-ABL tyrosine kinase in cellular lysis of human leukemia cell line. Consequently DNAzymes can be expected to act effectively in cellular system and also in vivo system.

MeSH terms

  • Base Sequence
  • DNA, Catalytic / chemical synthesis
  • DNA, Catalytic / chemistry*
  • Fusion Proteins, bcr-abl
  • Humans
  • Leukemia / enzymology
  • Peptides / chemistry
  • Protein-Tyrosine Kinases / antagonists & inhibitors
  • Tumor Cells, Cultured

Substances

  • DNA, Catalytic
  • Peptides
  • Protein-Tyrosine Kinases
  • Fusion Proteins, bcr-abl