Site-selective DNA hydrolysis by the combination of Ce(IV) and oligonucleotide bearing EDTA groups

Hiroyuki Arishima; Masahito Yokoyama; Makoto Komiyama

doi:10.1093/nass/3.1.137

Site-selective DNA hydrolysis by the combination of Ce(IV) and oligonucleotide bearing EDTA groups

Nucleic Acids Res Suppl. 2003:(3):137-8. doi: 10.1093/nass/3.1.137.

Authors

Hiroyuki Arishima¹, Masahito Yokoyama, Makoto Komiyama

Affiliation

¹ Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

PMID: 14510418
DOI: 10.1093/nass/3.1.137

Abstract

By using appropriately modified oligonucleotides, a gap-structure is formed in substrate DNA, and 2-6 ethylenediamine-N,N,N'-triacetate residues are placed near this gap. When these composites are treated with homogenous Ce(IV)/EDTA complex, the phosphodiester linkages in the gap-site are selectively hydrolyzed at much greater rates than achieved previously by using unmodified oligonucelotides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cerium / chemistry*
DNA / chemistry*
Edetic Acid / chemistry*
Hydrolysis
Molecular Sequence Data
Oligonucleotides / chemistry*

Substances

Oligonucleotides
Cerium
DNA
Edetic Acid