Abstract
This study demonstrates the usefulness of PLA and nested-PCR methods for the detection of cattle infection with enzootic bovine leukemia virus. Serological control with PLA was by 1.7% more sensitive than reference ELISA examination. Both the DNA of env-BLV gene in NCR, V-NCR, FLK and in recombinated AG clone cells, as well as in the whole blood and in blood isolated leukocytes of dairy cattle, were detected with the nested-PCR.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Animals
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Antibodies, Viral / blood
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Cattle
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DNA, Viral / blood
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Dairying
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Enzootic Bovine Leukosis / blood
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Enzootic Bovine Leukosis / diagnosis*
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Enzootic Bovine Leukosis / virology
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Enzyme-Linked Immunosorbent Assay / veterinary
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Female
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Immunodiffusion / veterinary
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Leukemia Virus, Bovine / immunology*
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Leukemia Virus, Bovine / isolation & purification
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Polymerase Chain Reaction / standards
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Polymerase Chain Reaction / veterinary*
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Predictive Value of Tests
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Sensitivity and Specificity
Substances
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Antibodies, Viral
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DNA, Viral