Abstract
[structure: see text] The interaction between the HIV-1 Tat protein and the TAR RNA element in the nascent viral genomic transcript is required for viral replication. An 11-residue beta-peptide (1), an all-beta homologue of the Arg-rich region Tat 47-57, binds TAR RNA with K(d) = 29 +/- 4 nM. A control beta-peptide (2) in which all Arg side chains are replaced by Lys side chains shows increased affinity but decreased specificity for wild-type vs bulge-deleted TAR RNA, as do the alpha-peptide analogues of 1 and 2.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Fluorescence Polarization
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Gene Products, tat / chemistry
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Gene Products, tat / genetics
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Gene Products, tat / metabolism*
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HIV Long Terminal Repeat
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HIV-1 / genetics
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Kinetics
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Oligopeptides / chemistry
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Oligopeptides / genetics
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Oligopeptides / metabolism*
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Protein Binding
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Protein Structure, Secondary
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RNA, Viral / metabolism*
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, tat
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Oligopeptides
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RNA, Viral
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tat Gene Products, Human Immunodeficiency Virus