Selective binding of TAR RNA by a Tat-derived beta-peptide

Org Lett. 2003 Oct 2;5(20):3563-5. doi: 10.1021/ol034977v.

Abstract

[structure: see text] The interaction between the HIV-1 Tat protein and the TAR RNA element in the nascent viral genomic transcript is required for viral replication. An 11-residue beta-peptide (1), an all-beta homologue of the Arg-rich region Tat 47-57, binds TAR RNA with K(d) = 29 +/- 4 nM. A control beta-peptide (2) in which all Arg side chains are replaced by Lys side chains shows increased affinity but decreased specificity for wild-type vs bulge-deleted TAR RNA, as do the alpha-peptide analogues of 1 and 2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Fluorescence Polarization
  • Gene Products, tat / chemistry
  • Gene Products, tat / genetics
  • Gene Products, tat / metabolism*
  • HIV Long Terminal Repeat
  • HIV-1 / genetics
  • Kinetics
  • Oligopeptides / chemistry
  • Oligopeptides / genetics
  • Oligopeptides / metabolism*
  • Protein Binding
  • Protein Structure, Secondary
  • RNA, Viral / metabolism*
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, tat
  • Oligopeptides
  • RNA, Viral
  • tat Gene Products, Human Immunodeficiency Virus