Abstract
We previously isolated a gene encoding thioltransferase (TTase1) from the fission yeast Schizosaccharomyces pombe. Using a TTase-lacZ fusion plasmid, carrying a 666 bp region upstream of the translation initiation point, we found that expression of TTase1 was enhanced by metal ions, diamide and NO-generating S-nitroso-N-acetylpenicillamine (SNAP). In the present work, we examined the regulation of TTase1 expression using a series of deletion mutants and identified a negatively acting sequence between bp -469 and -339. Atf1 is required for basal expression of TTase1, and Pap1 is required for its inducible expression by mercuric chloride, diamide and SNAP. The -469 approximately -339 bp region is also responsible for mediating the inducible expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activating Transcription Factor 1
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins / metabolism*
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Diamide / metabolism
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Drug Resistance / physiology
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Enzyme Inhibitors / metabolism
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Fungal Proteins / metabolism*
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Gene Expression Regulation, Fungal*
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Glutaredoxins
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Mercuric Chloride / metabolism
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Oxidoreductases / genetics*
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Oxidoreductases / metabolism
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Pancreatitis-Associated Proteins
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Phosphoproteins / metabolism*
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Plasmids / genetics
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Plasmids / metabolism
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S-Nitroso-N-Acetylpenicillamine / metabolism
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / metabolism
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Schizosaccharomyces pombe Proteins / metabolism*
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Sulfhydryl Reagents / metabolism
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Activating Transcription Factor 1
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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Enzyme Inhibitors
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Fungal Proteins
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Glutaredoxins
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Pancreatitis-Associated Proteins
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Pap1 protein, S pombe
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Phosphoproteins
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REG3A protein, human
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Schizosaccharomyces pombe Proteins
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Sulfhydryl Reagents
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atf1 protein, S pombe
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Diamide
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Mercuric Chloride
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S-Nitroso-N-Acetylpenicillamine
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Oxidoreductases
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beta-Galactosidase