Abstract
G protein-mediated pathways are the most fundamental mechanisms of cell signaling. In order to analyze these pathways, the availability of purified recombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.
MeSH terms
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Animals
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Baculoviridae / genetics
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Cell Line
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Chromatography / methods*
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GTP-Binding Protein alpha Subunits / genetics
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GTP-Binding Protein alpha Subunits / isolation & purification
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GTP-Binding Protein beta Subunits / genetics
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GTP-Binding Protein beta Subunits / isolation & purification
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GTP-Binding Protein gamma Subunits / genetics
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GTP-Binding Protein gamma Subunits / isolation & purification
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Heterotrimeric GTP-Binding Proteins / genetics
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Heterotrimeric GTP-Binding Proteins / isolation & purification*
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Histidine / metabolism
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Insecta
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification*
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Signal Transduction
Substances
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GTP-Binding Protein alpha Subunits
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GTP-Binding Protein beta Subunits
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GTP-Binding Protein gamma Subunits
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Recombinant Proteins
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Histidine
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Heterotrimeric GTP-Binding Proteins