A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach

Nucleic Acids Res. 2003 Oct 1;31(19):e115. doi: 10.1093/nar/gng113.

Abstract

A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotin- terminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allele- specific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotinylation
  • DNA Primers
  • DNA Transposable Elements
  • DNA, Plant / analysis
  • Fluorescent Dyes
  • Genetic Markers
  • Genotype
  • Oligonucleotide Array Sequence Analysis / economics
  • Oligonucleotide Array Sequence Analysis / methods*
  • Oligonucleotide Probes
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA / methods*

Substances

  • DNA Primers
  • DNA Transposable Elements
  • DNA, Plant
  • Fluorescent Dyes
  • Genetic Markers
  • Oligonucleotide Probes