Serum amyloid P component binding to Shiga toxin 2 requires both a subunit and B pentamer

Paola Marcato; Kathleen Vander Helm; George L Mulvey; Glen D Armstrong

doi:10.1128/IAI.71.10.6075-6078.2003

Serum amyloid P component binding to Shiga toxin 2 requires both a subunit and B pentamer

Infect Immun. 2003 Oct;71(10):6075-8. doi: 10.1128/IAI.71.10.6075-6078.2003.

Authors

Paola Marcato¹, Kathleen Vander Helm, George L Mulvey, Glen D Armstrong

Affiliation

¹ Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.

Abstract

Solid-phase binding, competitive binding, and cytotoxicity neutralization assays indicate that the B pentamer and A subunit both contribute to human serum amyloid P (HuSAP) component binding to Stx2. A polyvalent globotriaosyl-ceramide receptor analog, Daisy, did not competitively inhibit HuSAP binding, implying that the two ligands bind to different Stx2 domains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Binding, Competitive
Cell Line
Humans
In Vitro Techniques
Ligands
Neutralization Tests
Oligosaccharides / metabolism
Protein Structure, Tertiary
Protein Subunits
Serum Amyloid P-Component / metabolism*
Shiga Toxin 1 / chemistry
Shiga Toxin 1 / metabolism
Shiga Toxin 2 / chemistry
Shiga Toxin 2 / metabolism*

Substances

Daisy carbohydrate
Ligands
Oligosaccharides
Protein Subunits
Serum Amyloid P-Component
Shiga Toxin 1
Shiga Toxin 2