Isolation of a novel nuclear glycoprotein from pig kidney

Biochem Int. 1992 Sep;27(6):1001-9.

Abstract

A nuclear glycoprotein with an apparent Mr of 66,000 Da has been isolated from pig kidney chromatin after extraction with urea, guanidine-HCl and 2 M NaCl, and some of its structural features have been characterized. It belongs to the group of N-glycosylated proteins, which in the nucleus has so far received little attention. From its monosaccharide composition and recognition by lectins its oligosaccharides appear to be of high mannose and/or hybrid types. Some properties of its protein moiety suggest that it has a role in the packing of the DNA loops in the condensed chromatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Carbohydrate Sequence
  • Carbohydrates / analysis
  • Chromatin / chemistry
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Glycoproteins / genetics
  • Glycoproteins / isolation & purification*
  • Glycoproteins / metabolism
  • Kidney / chemistry*
  • Lectins / metabolism
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Nuclear Proteins / isolation & purification*
  • Nuclear Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Structure-Activity Relationship
  • Swine

Substances

  • Amino Acids
  • Carbohydrates
  • Chromatin
  • Glycoproteins
  • Lectins
  • Nuclear Proteins