ISOLATION AND CHARACTERIZATION OF A TOXIC MOIETY OF LOW MOLECULAR WEIGHT FROM CLOSTRIDIUM BOTULINUM TYPE A

J Bacteriol. 1965 May;89(5):1383-6. doi: 10.1128/jb.89.5.1383-1386.1965.

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, and Hardial S. Bains. Isolation and characterization of a toxic moiety of low molecular weight from Clostridium botulinum type A. J. Bacteriol. 89:1383-1386. 1965.-A toxic moiety of low molecular weight has been isolated from a type A strain of Clostridium botulinum, by a method involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. By means of electrophoresis and ultracentrifugation, the toxic substance was shown to be homogeneous; a molecular weight of 12,200 was calculated.

MeSH terms

  • Ammonium Compounds*
  • Animals
  • Antitoxins*
  • Biological Assay*
  • Canada
  • Cellulose*
  • Chemical Precipitation*
  • Chromatography*
  • Clostridium botulinum type A*
  • Clostridium botulinum*
  • DEAE-Cellulose*
  • Electrophoresis*
  • Hydrogen-Ion Concentration*
  • Mice
  • Molecular Weight*
  • Quaternary Ammonium Compounds*
  • Research*
  • Sulfates*
  • Toxins, Biological*
  • Ultracentrifugation*

Substances

  • Ammonium Compounds
  • Antitoxins
  • Quaternary Ammonium Compounds
  • Sulfates
  • Toxins, Biological
  • Cellulose
  • DEAE-Cellulose