Studies of the molecular recognition of synthetic methyl beta-lactoside analogues by Ricinus communis agglutinin

Carbohydr Res. 1992 Aug 3;232(2):207-26. doi: 10.1016/0008-6215(92)80055-6.

Abstract

The 2-, 3-, 6-, 2'-, 3'-, 4'-, and 6'-deoxy derivatives and the 3-O-methyl derivative of methyl beta-lactoside have been synthesised and their binding to the galactose-specific agglutinin from Ricinus communis (RCA-120) has been investigated. The results indicate that HO-3,4,6 of the beta-D-galactopyranose moiety are the key polar groups. The main difference from the closely related ricin lectin RCA-60 involves HO-6 of the D-glucopyranose moiety, which seems to contribute to the binding of the carbohydrate to RCA-60 but not to RCA-120.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Lactose / analogs & derivatives*
  • Lectins / chemistry*
  • Macromolecular Substances
  • Methylglycosides / chemistry*
  • Molecular Sequence Data
  • Plant Lectins
  • Plants, Toxic*
  • Ricinus communis / chemistry*
  • Solubility

Substances

  • Lectins
  • Macromolecular Substances
  • Methylglycosides
  • Plant Lectins
  • Ricinus communis agglutinin-1
  • methyl lactoside
  • Lactose