Calcium ionophore A-23187 inhibits the secretion of beta-hexosaminidase from the GG2EE mouse macrophage cell line

Biochem Int. 1992 Aug;27(5):783-91.

Abstract

Secretion of the lysosomal enzyme beta-N-acetylhexosaminidase is inhibited by calcium ionophore A-23187 in the GG2EE macrophage cell line. Such inhibition is time and dose dependent. Calcium ionophore A-23187 treatment causes a change in the pattern of hexosaminidase isoenzymes detectable in the cell extract, as assessed by DEAE-cellulose chromatography. In particular, control cells show two hexosaminidase isoenzymes corresponding to hexosaminidase A and B, whereas cells treated with calcium ionophore A-23187 express a third isoenzyme form with properties similar to hexosaminidase S.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcimycin / pharmacology*
  • Cell Line
  • Chromatography, DEAE-Cellulose
  • Hexosaminidase A
  • Isoenzymes / drug effects*
  • Isoenzymes / metabolism
  • Macrophages / drug effects*
  • Macrophages / enzymology
  • Mice
  • beta-N-Acetylhexosaminidases / drug effects*
  • beta-N-Acetylhexosaminidases / metabolism

Substances

  • Isoenzymes
  • Calcimycin
  • Hexosaminidase A
  • beta-N-Acetylhexosaminidases