Functional analysis of pAL5000 plasmid in Mycobacterium fortuitum

C A Villar; J Benitez

doi:10.1016/0147-619x(92)90047-e

Functional analysis of pAL5000 plasmid in Mycobacterium fortuitum

Plasmid. 1992 Sep;28(2):166-9. doi: 10.1016/0147-619x(92)90047-e.

Authors

C A Villar¹, J Benitez

Affiliation

¹ Centro Nacional de Investigaciones Cientificas, La Habana, Cuba.

PMID: 1409973
DOI: 10.1016/0147-619x(92)90047-e

Abstract

Four of the five open reading frames (ORFs) present in Myobacterium fortuitum pAL5000 plasmid (ORF1, ORF3, ORF4, and ORF5) are dispensable for replication in M. fortuitum. However, two additional ORFs (ORF1 and ORF5) were necessary for replication in a Myobacterium smegmatis heterologous host containing an efficient plasmid transformation mutation.

MeSH terms

DNA Replication
DNA, Bacterial / genetics
Nontuberculous Mycobacteria / genetics*
Open Reading Frames
Plasmids*
Species Specificity
Transformation, Bacterial

Substances

DNA, Bacterial