Phenylalanyl-tRNA synthetase from Thermus thermophilus can attach two molecules of phenylalanine to tRNA(Phe)

FEBS Lett. 1992 Oct 26;311(3):192-4. doi: 10.1016/0014-5793(92)81099-8.

Abstract

Phenylalanyl-tRNA synthetase from the extreme thermophilic bacterium Thermus thermophilus can incorporate more than one molecule of phenylalanine into the tRNA(Phe). It is shown that the 'hyperaminoacylated' tRNA(Phe) is the bis-2',3'-O-phenylalanyl-tRNA(Phe), and its formation is typical for the thermophilic enzyme but does not occur for E. coli phenylalanyl-tRNA synthetase under the same conditions.

Publication types

  • Comparative Study

MeSH terms

  • Autoradiography / methods
  • Carbon Radioisotopes
  • Chromatography, Thin Layer
  • Kinetics
  • Phenylalanine-tRNA Ligase / metabolism*
  • Protein Binding
  • RNA, Transfer, Phe / isolation & purification
  • RNA, Transfer, Phe / metabolism*
  • Radioisotope Dilution Technique
  • Thermus thermophilus / enzymology*

Substances

  • Carbon Radioisotopes
  • RNA, Transfer, Phe
  • Phenylalanine-tRNA Ligase