Homeostasis as regulated by activated macrophage. VIII. LPSw (a lipopolysaccharide from wheat flour) can regulate bone resorption of chick embryo

K Kawashima; H Endo; T Nishizawa; H Inagawa; T Okutomi; A Morikawa; G Soma; D Mizuno

doi:10.1248/cpb.40.1271

Homeostasis as regulated by activated macrophage. VIII. LPSw (a lipopolysaccharide from wheat flour) can regulate bone resorption of chick embryo

Chem Pharm Bull (Tokyo). 1992 May;40(5):1271-3. doi: 10.1248/cpb.40.1271.

Authors

K Kawashima¹, H Endo, T Nishizawa, H Inagawa, T Okutomi, A Morikawa, G Soma, D Mizuno

Affiliation

¹ Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.

PMID: 1394647
DOI: 10.1248/cpb.40.1271

Abstract

The effect of LPSw (a lipopolysaccharide from wheat flour) on the bone resorption of 18-d chick embryonic calvaria was examined in an organ culture following the method of Raisz. Bone was prelabeled in culture medium containing 45Ca and chased in a cold medium. On addition of test samples, labeled calcium was released indicating the grade of bone resorption. LPSw (10-100 ng/ml) stimulated bone resorption, showing an effect comparable to parathyroid hormone (PTH) (1 U/ml). PTH at 1 U/ml decreased the total amount of calcium and phosphorus, while LPSw did not. LPSw is thus assumed to stimulate bone resorption more actively than PTH.

MeSH terms

Animals
Bone Resorption / drug therapy*
Bone Resorption / physiopathology
Chick Embryo
Homeostasis / drug effects
Homeostasis / physiology*
Lipopolysaccharides / therapeutic use*
Macrophage Activation / drug effects
Macrophage Activation / physiology*
Macrophages / drug effects
Macrophages / physiology
Organ Culture Techniques
Skull / embryology
Skull / physiopathology
Triticum

Substances

Lipopolysaccharides