Abstract
E. coli strain A49 carries the themosensitive mutation in the rnpA gene encoding the protein component of RNase P, a tRNA-processing enzyme. Two small RNAs were highly accumulated in the A49 carrying derivatives of ColE1-type plasmids, at nonpermissive temperature. Characterization of these RNAs showed that they were the processed or degraded products derived from RNA I, which is the negative controller of ColE1-type plasmid replication. These derivatives of RNA I only differ in size at the 5' ends. The data of their degradation and synthesis kinetics suggest that they are intermediates of RNA I metabolism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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DNA, Bacterial / metabolism
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Endoribonucleases / genetics*
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins*
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Genes, Bacterial
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Molecular Sequence Data
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Plasmids
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RNA, Bacterial / genetics
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RNA, Bacterial / isolation & purification
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RNA, Bacterial / metabolism*
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RNA, Catalytic / genetics*
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Ribonuclease P
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Temperature
Substances
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DNA, Bacterial
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Escherichia coli Proteins
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RNA I
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RNA, Bacterial
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RNA, Catalytic
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Endoribonucleases
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Ribonuclease P
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ribonuclease P, E coli
Associated data
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GENBANK/D10916
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GENBANK/D10917
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GENBANK/D10918
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GENBANK/D10919
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GENBANK/D10920
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GENBANK/S39220
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GENBANK/S39221
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GENBANK/S42971
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GENBANK/S42972
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GENBANK/S42973