Abstract
The extraction of Penicillin G (PG) from the filtered cultivation medium of Penicillium chrysogenum and its conversion into 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PhA) at pH 8 was performed in a 10 l kühni extractor during the production by means of penicillin-G-amidase immobilized in a liquid membrane carrier system (LM). 6-APA was enriched in LM, and the PhA returned to the cultivation medium. After electrocoalescence of LM, the 6-APA was converted into ampicillin with the same enzyme at pH 6, while the liquid membrane phase and enzyme were recycled and reused.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ampicillin / isolation & purification
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Ampicillin / metabolism
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Biotransformation
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Culture Media
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Enzymes, Immobilized
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Membranes, Artificial
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Penicillanic Acid / metabolism
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Penicillins / biosynthesis*
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Penicillins / isolation & purification
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Penicillins / pharmacokinetics*
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Penicillium chrysogenum / chemistry
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Penicillium chrysogenum / growth & development
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Penicillium chrysogenum / metabolism*
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Permeability
Substances
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Culture Media
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Enzymes, Immobilized
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Membranes, Artificial
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Penicillins
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Ampicillin
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Penicillanic Acid
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aminopenicillanic acid