Evidence of introduction by molecular cloning of artificial inverted sequence at the 5' terminus of the sense strand of rat insulin-like growth factor-I cDNA

H Kato; A Takenaka; Y Miura; M Nishiyama; T Noguchi

Evidence of introduction by molecular cloning of artificial inverted sequence at the 5' terminus of the sense strand of rat insulin-like growth factor-I cDNA

Agric Biol Chem. 1990 Sep;54(9):2225-30.

Authors

H Kato¹, A Takenaka, Y Miura, M Nishiyama, T Noguchi

Affiliation

¹ Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.

PMID: 1368576

Abstract

A cDNA of insulin-like growth factor (IGF)-I mRNA was obtained from a rat liver cDNA library. This cDNA contained a 5' terminal sequence of 48 base pairs complementary to a sequence in the 3' terminal region (an inverted repeat sequence) except for one nucleotide insertion in the 3' region. Base sequence analysis of this cDNA, northern blot analysis of rat liver mRNA using a probe specific to 5' region of cDNA, base sequence studies of the 5' upstream region of a clone of the rat IGF-I gene, and some other studies suggested strongly that the inverted repeat sequence of the IGF-I cDNA used was introduced artificially during preparation of the cDNA library.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular*
DNA / genetics
DNA Probes
Exons
Insulin-Like Growth Factor I / genetics*
Introns
Liver / chemistry
Molecular Sequence Data
Nucleic Acid Hybridization
RNA, Messenger / genetics
Rats
Repetitive Sequences, Nucleic Acid

Substances

DNA Probes
RNA, Messenger
Insulin-Like Growth Factor I
DNA