A new cDNA clone relating to larger molecular species of rat insulin-like growth factor-I mRNA

Agric Biol Chem. 1990 Jun;54(6):1599-601.

Abstract

A new cDNA clone for rat IGF-I mRNA was obtained. The cDNA contained a new base sequence as cDNA in the 3' region. The sequence coincided with a part of the sequence reported earlier by Shimatsu and Rotwein in exon 5 of the rat IGF-I gene. The presence of this cDNA proved the previous suggestion to be true that the size heterogeneity of IGF-I mRNA is primarily due to the heterogeneity of the 3'-untranslated region.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA / genetics*
  • Exons
  • Insulin-Like Growth Factor I / genetics*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics*
  • Rats

Substances

  • RNA, Messenger
  • Insulin-Like Growth Factor I
  • DNA