Antioxidant effect of flavonoids on DCF production in HL-60 cells

Phytother Res. 2003 Sep;17(8):963-6. doi: 10.1002/ptr.1289.

Abstract

A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use
  • Dose-Response Relationship, Drug
  • Flavonoids / administration & dosage
  • Flavonoids / pharmacology*
  • Flavonoids / therapeutic use
  • Flow Cytometry
  • Fluoresceins / chemistry
  • HL-60 Cells / drug effects
  • Humans
  • Oxidation-Reduction
  • Phytotherapy*
  • Plants, Medicinal*
  • Respiratory Burst / drug effects*

Substances

  • Antioxidants
  • Flavonoids
  • Fluoresceins
  • diacetyldichlorofluorescein