Abstract
A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated.
Copyright 2003 John Wiley & Sons, Ltd.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antioxidants / administration & dosage
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Antioxidants / pharmacology*
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Antioxidants / therapeutic use
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Dose-Response Relationship, Drug
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Flavonoids / administration & dosage
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Flavonoids / pharmacology*
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Flavonoids / therapeutic use
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Flow Cytometry
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Fluoresceins / chemistry
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HL-60 Cells / drug effects
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Humans
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Oxidation-Reduction
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Phytotherapy*
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Plants, Medicinal*
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Respiratory Burst / drug effects*
Substances
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Antioxidants
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Flavonoids
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Fluoresceins
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diacetyldichlorofluorescein