In differentiation division, the mitosis occurring within all tissues consists of more than one type of mitosis. It occurs as two antagonistic types: the essential duplication mitosis activated by the effect of duplication factor and the antagonistic maturation mitosis which develops from the essential type and is further stimulated by maturation factor. These different types of mitosis play antagonistic roles in histological development, while maintaining a specified physio-mitotic balance through their respective mitotic factors and mitotic regulatory mechanisms in the tissues. Depending on the physio-mitotic balance between duplication and maturation mitosis, each layer of organized tissue, comprised of intrinsic functional cells, differentiates from one of the three germ layers, without errors that create abnormal characteristics. In this way, the tissues establish histological identity and continuity while maintaining a prescribed histological organization without excess or reduced multiplication within the tissues. This forms the fundamental basis of the physio-mitotic theory.