Adenylate cyclase from sea urchin eggs is positively and negatively regulated by D-1 and D-2 dopamine receptors

Exp Cell Res. 1992 Dec;203(2):491-4. doi: 10.1016/0014-4827(92)90026-5.

Abstract

The adenylate cyclase present in membranes prepared from sea urchin eggs is sensitive to dopamine stimulation. The receptor sites coupled to sea urchin adenylate cyclase were characterized by means of specific agonists and antagonists. The D-1 dopamine agonist SKF-38393 was able to stimulate enzyme activity, while the two D-1 dopamine antagonists, SCH-23390 and SKF-83566, suppressed the stimulatory effect of dopamine. In addition, the D-2 dopamine agonists, PPHT and metergoline, brought about a dose-dependent inhibition of dopamine-stimulated adenylate cyclase activity. These data show that: (i) in sea urchin eggs adenylate cyclase is regulated by dopamine receptors; (ii) these receptors share characteristics with D-1 and D-2 dopamine receptors present in the mammalian brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / analogs & derivatives
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / pharmacology
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Benzazepines / pharmacology
  • Dopamine / pharmacology
  • Dopamine Agents / pharmacology
  • Metergoline / pharmacology
  • Ovum / enzymology*
  • Phenethylamines / pharmacology
  • Receptors, Dopamine D1 / metabolism*
  • Receptors, Dopamine D2 / metabolism*
  • Sea Urchins

Substances

  • Benzazepines
  • Dopamine Agents
  • Phenethylamines
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2
  • Metergoline
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
  • 2-(N-phenethyl-N-propyl)amino-5-hydroxytetralin
  • 1H-3-benzazepin-7-ol, 8-bromo-2,3,4,5-tetrahydro-3-methyl-5-phenyl-
  • Adenylyl Cyclases
  • Dopamine