Amplification and direct sequencing of a cDNA encoding human cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase

Biochim Biophys Acta. 1992 Oct 20;1132(3):329-31. doi: 10.1016/0167-4781(92)90172-v.

Abstract

Cytosolic 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) synthase (E.C. 4.1.3.5) is a highly regulated enzyme involved in isoprenoid biosynthesis and therefore a potential target for cholesterol-lowering drugs. Up to now, primary structure data have only been available for chicken, rat and hamster HMG-CoA synthase. Using in vitro amplification and direct sequencing, we have determined the nucleotide sequence of the coding region of the human cytosolic 3-hydroxy-3-methylglutaryl CoA synthase cDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA
  • Humans
  • Hydroxymethylglutaryl-CoA Synthase / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction

Substances

  • DNA
  • Hydroxymethylglutaryl-CoA Synthase

Associated data

  • GENBANK/L07316
  • GENBANK/M91429
  • GENBANK/M91430
  • GENBANK/M93297
  • GENBANK/M93298
  • GENBANK/M93299
  • GENBANK/M93300
  • GENBANK/M93301
  • GENBANK/M94056
  • GENBANK/X66435