Streptococcal pyrogenic exotoxin A and streptolysin O enhance polymorphonuclear leukocyte binding to gelatin matrixes

J Infect Dis. 1992 Jul;166(1):165-9. doi: 10.1093/infdis/166.1.165.

Abstract

Autopsy data from cases of streptococcal toxic shock demonstrate accumulation of polymorphonuclear leukocytes (PMNL) within lung and soft tissue microvasculature. Because of the increased prevalence of streptococcal pyrogenic exotoxin A (SPEA)-producing strains associated with streptococcal toxic shock syndrome, experiments were done to determine whether SPEA or streptolysin O (SLO, a thiol-activated cytolysin produced by all group A streptococci) could stimulate PMNL-dependent adherence mechanisms in vitro. SPEA (0.01-10 micrograms/5.5 x 10(6) PMNL) only modestly enhanced PMNL adherence over the entire range of concentrations tested. In contrast, SLO-induced PMNL binding was highly dose dependent (maximal binding, 55.1 +/- 1.6% at 0.5 hemolytic units/5.5 x 10(6) PMNL) and was mediated by CD11/CD18 adherence glycoprotein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antigens, CD / physiology
  • Bacterial Proteins
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion / drug effects
  • Cells, Cultured
  • Exotoxins / pharmacology*
  • Humans
  • Membrane Proteins*
  • Neutrophils / drug effects
  • Neutrophils / metabolism*
  • Pyrogens / pharmacology*
  • Receptors, Leukocyte-Adhesion / physiology
  • Streptolysins / pharmacology*

Substances

  • Antigens, CD
  • Bacterial Proteins
  • CD11 Antigens
  • CD18 Antigens
  • Exotoxins
  • Membrane Proteins
  • Pyrogens
  • Receptors, Leukocyte-Adhesion
  • SpeA protein, Streptococcus pyogenes
  • Streptolysins
  • erythrogenic toxin
  • streptolysin O