Repression of the viral latent promoter BC-R2 in Epstein-Barr virus negative cell lines

Biochem Biophys Res Commun. 1992 Dec 30;189(3):1695-700. doi: 10.1016/0006-291x(92)90273-n.

Abstract

The regulation of the latent promoter BC-R2 of Epstein-Barr virus (EBV) was examined using the chloramphenicol acetyl transferase (CAT) gene reporter system. A 5' deletion analysis of BC-R2 promoter sequences has been used to characterize a region, described previously as a transcriptional enhancer in EBV positive cell lines, which can repress the BC-R2 transcriptional activity in EBV negative cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Burkitt Lymphoma
  • Cell Line
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism*
  • DNA Replication
  • Enhancer Elements, Genetic
  • Exons
  • Gene Deletion
  • Gene Expression Regulation, Viral*
  • Herpesvirus 4, Human / genetics*
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Plasmids
  • Promoter Regions, Genetic*
  • Recombinant Fusion Proteins / metabolism
  • Transcription, Genetic
  • Tumor Cells, Cultured

Substances

  • Recombinant Fusion Proteins
  • Chloramphenicol O-Acetyltransferase