Assignment of the human CRABP-II gene to chromosome 1q21 by nonisotopic in situ hybridization

Hum Genet. 1992 Jul;89(5):487-90. doi: 10.1007/BF00219171.

Abstract

Two highly conserved forms of cellular retinoic acid binding protein (CRABP-I and CRABP-II) have been described, and one, CRABP-II, is highly expressed in human skin. We have utilized a 10-kb fragment containing the human CRABP-II (hCRABP-II) gene (isolated from a human genomic library) to localize hCRABP-II to human chromosome 1 band q21 by fluorescence in situ hybridization. Localization to 1q was confirmed by hybridization of a hCRABP-II cDNA clone against a human-mouse hybrid cell line containing a t(1;6)(q21;q13)) translocation chromosome. The hCRABP-II gene is therefore localized to a band known to contain several other genes that are expressed in the context of epidermal differentiation, including profilaggrin, loricrin, involucrin, and calcyclin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carrier Proteins / genetics*
  • Cell Differentiation
  • Chromosome Mapping / methods*
  • Chromosomes, Human, Pair 1*
  • DNA Probes
  • Genes / genetics*
  • Humans
  • Hybrid Cells
  • Keratinocytes / cytology
  • Mice
  • Microscopy, Fluorescence
  • Nucleic Acid Hybridization
  • Receptors, Retinoic Acid
  • Translocation, Genetic
  • Tretinoin*

Substances

  • Carrier Proteins
  • DNA Probes
  • Receptors, Retinoic Acid
  • Tretinoin