Abstract
The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which is interrupted by a 68 bp long intron. The putative Swi4 protein shows homology to Duc-1 (human), Rep-3 (mouse), HexA (Streptococcus pneumoniae) and MutS (Salmonella typhimurium). The prokaryotic proteins are known as essential components involved in mismatch repair. A strain with a disrupted swi4+ gene was constructed and analysed with respect to the switching process. As in swi4 mutants duplications occur in the mating-type region of the swi4 (null) strain, reducing the efficiency of switching.
MeSH terms
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Adenosine Triphosphatases*
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Amino Acid Sequence
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Animals
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Bacterial Proteins / chemistry
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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DNA Repair / genetics*
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DNA-Binding Proteins*
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Escherichia coli Proteins*
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Genes, Fungal*
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Genes, Mating Type, Fungal*
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Genes, Switch*
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Humans
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Molecular Sequence Data
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MutS DNA Mismatch-Binding Protein
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Restriction Mapping
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Sequence Homology, Nucleic Acid
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Transcription Factors*
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Escherichia coli Proteins
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Fungal Proteins
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SWI4 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Adenosine Triphosphatases
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MutS DNA Mismatch-Binding Protein
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MutS protein, E coli