In vitro inhibition of 5-lipoxygenase metabolite, leukotriene B4, in bovine mononuclear cells by bovine viral diarrhea virus

Vet Immunol Immunopathol. 1992 Feb 15;31(1-2):49-59. doi: 10.1016/0165-2427(92)90086-6.

Abstract

Bovine viral diarrhea (BVD) virus inhibited phytohemagglutinin (PHA)-, PHA plus phorbol-12-myristate-13 acetate (PMA)- or PHA plus calcium ionophore (A23187)-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation. Further, BVD-virus inhibited A23187-stimulated leukotriene B4 (LTB4) synthesis into the culture supernatants. Presence of exogenous LTB4 failed to reverse the BVD virus-induced immunosuppression. Our results suggest that BVD virus-induced immunosuppression is due to a factor that may be necessary to induce LTB4 synthesis for normal mononuclear cell proliferation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachidonate 5-Lipoxygenase / metabolism*
  • Calcimycin / pharmacology
  • Cattle
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Diarrhea Viruses, Bovine Viral / physiology*
  • Immunosuppression Therapy
  • Leukocytes, Mononuclear / immunology*
  • Leukocytes, Mononuclear / metabolism
  • Leukocytes, Mononuclear / microbiology
  • Leukotriene B4 / antagonists & inhibitors*
  • Lymphocyte Activation
  • Mitogens / pharmacology
  • Radioimmunoassay

Substances

  • Mitogens
  • Leukotriene B4
  • Calcimycin
  • Arachidonate 5-Lipoxygenase