Abstract
Bovine viral diarrhea (BVD) virus inhibited phytohemagglutinin (PHA)-, PHA plus phorbol-12-myristate-13 acetate (PMA)- or PHA plus calcium ionophore (A23187)-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation. Further, BVD-virus inhibited A23187-stimulated leukotriene B4 (LTB4) synthesis into the culture supernatants. Presence of exogenous LTB4 failed to reverse the BVD virus-induced immunosuppression. Our results suggest that BVD virus-induced immunosuppression is due to a factor that may be necessary to induce LTB4 synthesis for normal mononuclear cell proliferation.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Arachidonate 5-Lipoxygenase / metabolism*
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Calcimycin / pharmacology
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Cattle
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Cells, Cultured
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Chromatography, High Pressure Liquid
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Diarrhea Viruses, Bovine Viral / physiology*
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Immunosuppression Therapy
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Leukocytes, Mononuclear / immunology*
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Leukocytes, Mononuclear / metabolism
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Leukocytes, Mononuclear / microbiology
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Leukotriene B4 / antagonists & inhibitors*
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Lymphocyte Activation
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Mitogens / pharmacology
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Radioimmunoassay
Substances
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Mitogens
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Leukotriene B4
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Calcimycin
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Arachidonate 5-Lipoxygenase