[The polymerase chain reaction (PCR) for the detection of DNA of equine herpesviruses 1 and 4]

Berl Munch Tierarztl Wochenschr. 1992 Feb 1;105(2):52-5.
[Article in German]

Abstract

Formalin-fixed and Paraplast-embedded tissue samples of 42 aborted equine fetuses were examined by polymerase chain reaction for the presence of equine herpesvirus DNA. The used set of primers was located in the glycoprotein 13 open reading frame and allowed the amplification of both EHV 1 und EHV 4. By cleaving pattern analysis after Hinf I digestion EHV 1 could be distinguished from EHV 4. In 9 of the cases investigated EHV 1-DNA was detected. This finding is in absolute context with the results of the virological investigations.

Publication types

  • English Abstract

MeSH terms

  • Abortion, Veterinary / microbiology
  • Animals
  • DNA, Viral / analysis*
  • Female
  • Herpesviridae / genetics*
  • Herpesviridae Infections / microbiology
  • Herpesviridae Infections / veterinary*
  • Herpesvirus 1, Equid / genetics*
  • Horse Diseases / microbiology*
  • Horses
  • Polymerase Chain Reaction
  • Pregnancy

Substances

  • DNA, Viral