Insulin-like system and growth regulation in the Pacific oyster Crassostrea gigas: hrIGF-1 effect on protein synthesis of mantle edge cells and expression of an homologous insulin receptor-related receptor

Gen Comp Endocrinol. 2003 Oct 15;134(1):44-56. doi: 10.1016/s0016-6480(03)00217-x.

Abstract

The involvement of molecules belonging to the insulin/IGF family in regulation of growth has been investigated in the Pacific oyster Crassostrea gigas. In vitro biological effects of human recombinant IGF-1 (hrIGF-1) on mantle edge cells, involved in oyster shell and soft body growth, were studied over an annual cycle. In mantle edge cells hrIGF-1 stimulates protein synthesis of 56+/-5.1% over basal for 10(-10) M in September with in addition a clear dose-effect corresponding to the highest shell growth period, and 57.5+/-3.45% over basal for 10(-11) M in March and 51+/-5.4% over basal for 10(-10) M in April corresponding to the period of mantle growth. These insulin-like effects were associated with the expression of a recently identified C. gigas insulin receptor-related receptor (CIR) in mantle edge cells as demonstrated by RT-PCR. Moreover, in situ hybridisation (ISH) confirmed this expression at the level of the inner and outer epithelia involved in mantle growth and shell formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Base Sequence
  • Epidermal Cells
  • Epidermis / growth & development
  • Epidermis / metabolism*
  • Humans
  • In Vitro Techniques
  • Insulin / physiology*
  • Insulin-Like Growth Factor I / physiology*
  • Membrane Proteins
  • Molecular Sequence Data
  • Organoids / cytology
  • Organoids / metabolism
  • Ostreidae / cytology
  • Ostreidae / growth & development*
  • Ostreidae / metabolism
  • Protein Biosynthesis
  • RNA, Messenger / analysis
  • Receptor, Insulin / genetics
  • Receptor, Insulin / metabolism*
  • Recombinant Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seasons

Substances

  • Insulin
  • Membrane Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • Insulin-Like Growth Factor I
  • Receptor, Insulin
  • insulin receptor-related receptor