Expression of herpes simplex virus type 1 glycoprotein B in insect cells. Initial analysis of its biochemical and immunological properties

Virus Res. 1992 Jan;22(1):25-39. doi: 10.1016/0168-1702(92)90087-p.

Abstract

A recombinant baculovirus (vAc-gB1) was constructed which expresses the glycoprotein B (gB) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with these recombinant viruses, a protein that was close in size to authentic HSV-1 gB was detected by gB polyclonal antibody. The recombinant gB was found on the membrane of Sf9 cells and was susceptible to tunicamycin, glycosidase F (PNGase F) and partially susceptible to Endo-H. Antibodies raised in mice to this recombinant recognized viral gB and neutralized the infectivity of HSV-1 in vitro. Mice inoculated with the recombinant gB were protected from lethal challenge with HSV-1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / immunology
  • Baculoviridae / genetics
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Viral
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Glycosylation
  • Herpes Simplex / prevention & control
  • Kinetics
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Moths
  • Neutralization Tests
  • Simplexvirus / immunology*
  • Tunicamycin / pharmacology
  • Vaccination
  • Vero Cells
  • Viral Envelope Proteins / biosynthesis*
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus
  • Tunicamycin