Abstract
A recombinant baculovirus (vAc-gB1) was constructed which expresses the glycoprotein B (gB) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with these recombinant viruses, a protein that was close in size to authentic HSV-1 gB was detected by gB polyclonal antibody. The recombinant gB was found on the membrane of Sf9 cells and was susceptible to tunicamycin, glycosidase F (PNGase F) and partially susceptible to Endo-H. Antibodies raised in mice to this recombinant recognized viral gB and neutralized the infectivity of HSV-1 in vitro. Mice inoculated with the recombinant gB were protected from lethal challenge with HSV-1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Viral / immunology
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Baculoviridae / genetics
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Base Sequence
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Blotting, Southern
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Cloning, Molecular
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DNA, Viral
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Enzyme-Linked Immunosorbent Assay
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Fluorescent Antibody Technique
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Glycosylation
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Herpes Simplex / prevention & control
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Kinetics
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Moths
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Neutralization Tests
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Simplexvirus / immunology*
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Tunicamycin / pharmacology
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Vaccination
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Vero Cells
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Viral Envelope Proteins / biosynthesis*
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Viral
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DNA, Viral
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Viral Envelope Proteins
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glycoprotein B, Simplexvirus
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Tunicamycin