Composition of lipid A in the S and R forms of Shigella dysenteriae serovar 1

Zentralbl Bakteriol. 1992 Dec;277(4):429-35. doi: 10.1016/s0934-8840(11)80467-6.

Abstract

A chemical analysis was performed of lipid A, isolated by acid hydrolysis of the lipopolysaccharide from the S and R forms of Shigella dysenteriae serovar 1. Differences in the moiety of both lipids and sugars were compared. The lipid portion consisted of a homologous series of fatty acids ranging from C12:0 to C18:0 (predominant homologues, C12:0, C14:0 and C16:0) and the homologous series of 3-hydroxy acids ranging from C12:0 to C16:0 (predominant homologue, 3-OH-C14:0). The major sugar portion consisted of D-glucosamine. The toxicity of lipid A in mice (LD50) ranged between 300-400 micrograms/mouse, and values from the Limulus amebocyte lysate assay were recorded as titres of 10(-5) to 10(-6) mg/ml.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Carbohydrates / analysis
  • Fatty Acids / analysis
  • Glucosamine / analysis
  • Lethal Dose 50
  • Limulus Test
  • Lipid A / chemistry*
  • Lipid A / toxicity
  • Mice
  • Mice, Inbred ICR
  • Serotyping
  • Shigella dysenteriae / chemistry*
  • Shigella dysenteriae / classification

Substances

  • Carbohydrates
  • Fatty Acids
  • Lipid A
  • Glucosamine