Six cultivars including strong, middle and weak gluten wheat varieties were cultivated at five latitudes(from 32 degrees N to 36 degrees N) in Henan Province, and the variations of their qualitative characters were analyzed in relation to the different environments. The result indicated that latitudes had great effects on the protein content, wet gluten content, softening degree, evaluation value, and extensibility of the cultivars. The rheological parameters measured by farinogram and the extensogram were significantly different among different among different cultivars, and the coefficients of variation were quite large. The variations in qualitative characters among cultivars and among latitudes were different. With the rising latitude, the protein content, wet gluten content, development time, evaluation value, extensibility, resistance, and maximum resistance showed an increase tendency, with a few exceptions for certain latitude. For most qualitative characters, there was a clear boundary between Xinyan(32 degrees N) and Zhumadian(33 degrees N). According to these results, suggestions in the latitude layout of the hi-quality wheat cultivars and in improving their cultuvation practices were made.