Dendritic cells (DCs) are the most potent antigen-presenting cells in vitro and in vivo. They play a key role in the initiation of the immune response and are considered promising targets for immunotherapy. The recent DC Keystone Symposia, organized by Ralph M Steinman, Anne O'Garra and Jacques Banchereau, was held on 3-8 March 2003 in Keystone (CO, USA). This multidisciplinary meeting developed various areas related to the DC biology including: (i) DC and the control of immunity vs tolerance; (ii) DC maturation and manipulation for immunotherapy in vivo; and (iii) antigen capture by DC in situ and interactions with microbial pathogens. The aim of this report is to present some of the highlights developed during the meeting and debated among the DC community.