Image analysis of light distribution in a photobioreactor

Biotechnol Bioeng. 2003 Nov 5;84(3):394-7. doi: 10.1002/bit.10766.

Abstract

Light intensity is a crucial factor that determines the growth of photosynthetic cells. This study analyzed the light distribution in a photobioreactor by processing images, captured with a digital camera, of a rectangular photobioreactor containing Synechococcus sp. PCC6801 as a model microorganism. The gray-scale images obtained clearly demonstrate the variation of the light-distribution profiles upon changing cell concentrations and external light intensity. Image-processing techniques were also used to predict the cell density in the photobioreactor. By analyzing the digitized image data with a neural network model, we were able to predict the cell concentrations in the photobioreactor with a <5% error.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Bioreactors / microbiology*
  • Cell Count / methods*
  • Cyanobacteria / cytology*
  • Image Interpretation, Computer-Assisted / methods*
  • Neural Networks, Computer*
  • Photobiology / instrumentation*
  • Photobiology / methods*
  • Photometry / methods*
  • Video Recording / methods