Abstract
A gene (pagA) encoding beta-agarase from Pseudomonas sp. SK38 was cloned and expressed in Escherichia coli. The structural gene consists of 1011 bp encoding 337 amino acids with a predicted molecular weight of 37326 and has a signal peptide of 18 amino acids. The deduced amino acid sequence showed 57% and 58% homology to beta-agarase from Pseudoalteromonas atalntica and Aeromonas sp., respectively. The recombinant enzyme was purified and biochemically characterized. The enzyme had maximum activity at pH 9 and 30 degrees C. It was stable at pHs from 8 to 9 and below 37 degrees C.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Glycoside Hydrolases / biosynthesis*
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Glycoside Hydrolases / chemistry
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / metabolism
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Molecular Sequence Data
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Pseudomonas / enzymology
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Pseudomonas / genetics*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Sequence Homology, Amino Acid
Substances
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DNA, Bacterial
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Recombinant Proteins
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Glycoside Hydrolases
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agarase