Abstract
The Schizosaccharomyces pombe rad51(+) and dmc1(+) genes code for homologues of the Escherichia coli recombination protein RecA. Deletion of rad51(+) causes slow growth, retardation of cell division and a decrease in viability. rad51Delta cells have a defect in mating-type switching. The DNA modification at the mating-type locus required for mating-type switching contributes to slow growth in the rad51 mutant. Cell mating is reduced in crosses homozygous for rad51Delta. Ectopic expression of the dmc1(+) gene allowed us to demonstrate that the reduction in meiotic recombination in dmc1 mutants is not caused by a disturbance of rad24 expression from the dmc1- rad24 bicistronic RNA. We describe the functional defects of terminally epitope-tagged Dmc1 and Rad51 and discuss it in terms of protein interaction. Presumptive Rad51 and Dmc1 foci were detected on spreads of meiotic chromatin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases*
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Animals
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Antibodies, Monoclonal
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Cell Compartmentation
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Cell Cycle Proteins / biosynthesis*
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Cell Cycle Proteins / genetics
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Crossing Over, Genetic
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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Epitopes
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Escherichia coli
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Gene Deletion
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Gene Expression Regulation, Fungal
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Genetic Vectors
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Green Fluorescent Proteins
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Immunochemistry
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Luminescent Proteins / biosynthesis
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Luminescent Proteins / genetics
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Mice
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Mice, Knockout
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Nuclear Proteins
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Phosphate-Binding Proteins
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Rad51 Recombinase
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Rec A Recombinases / genetics*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces / ultrastructure
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Schizosaccharomyces pombe Proteins*
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Sequence Homology, Nucleic Acid
Substances
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Antibodies, Monoclonal
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Cell Cycle Proteins
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DNA-Binding Proteins
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Dmc1 protein, mouse
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Epitopes
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Luminescent Proteins
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Nuclear Proteins
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Phosphate-Binding Proteins
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RHP51 protein, S pombe
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Recombinant Fusion Proteins
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Schizosaccharomyces pombe Proteins
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Green Fluorescent Proteins
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Rad51 Recombinase
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Rad51 protein, mouse
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Rec A Recombinases
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Adenosine Triphosphatases