Abstract
A synthetic beta-thio-fructofuranoside of mercaptoethanol inhibited not only beta-fructofuranosidases but also alpha-glucosidases. The compound was hardly hydrolyzed by the glycosidases. The thio-fructoside competitively inhibited beta-fructofuranosidases from Aspergillus niger, Candida sp., and Saccharomyces cerevisiae, but not Arthrobacter beta-fructofuranosidase at all. Sucrase activity of rat intestinal sucrase/isomaltase complex was also suppressed in the presence of the thio-fructoside. The thio-fructoside showed noncompetitive inhibition toward maltase activity of the rat intestinal enzyme complex and Saccharomyces sp. alpha-glucosidase. Inhibition against the Bacillus stearothermophilus alpha-glucosidase, Rhizopus glucoamylase, and porcine kidney trehalase were more slight than that against these two alpha-glucosidases.
MeSH terms
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Animals
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Arthrobacter / enzymology
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Aspergillus niger / enzymology
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Binding, Competitive
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Candida / enzymology
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / metabolism
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Enzyme Inhibitors / pharmacology
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Fructose / analogs & derivatives*
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Fructose / metabolism
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Fructose / pharmacology*
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Geobacillus stearothermophilus / enzymology
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Glucan 1,4-alpha-Glucosidase / metabolism
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Glycoside Hydrolase Inhibitors*
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Hydrolysis
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Intestines / enzymology
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Kinetics
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Maltose / metabolism
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Rats
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Rhizopus / enzymology
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Saccharomyces cerevisiae / enzymology
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Sucrase / metabolism
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Sucrose / metabolism
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Sulfides / chemistry
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Sulfides / metabolism
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Sulfides / pharmacology*
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Swine
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Trehalase / metabolism
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beta-Fructofuranosidase / antagonists & inhibitors*
Substances
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Enzyme Inhibitors
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Glycoside Hydrolase Inhibitors
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Sulfides
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Fructose
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Sucrose
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Maltose
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beta-Fructofuranosidase
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Trehalase
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Glucan 1,4-alpha-Glucosidase
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Sucrase