Abstract
Leukotriene B4 (LTB4) is a potent chemoattractant for myeloid leukocytes, which express BLT1, the high-affinity receptor for LTB4. We report here that BLT1 is induced substantially in CD8+ effector T cells and at lower amounts in CD8+ central memory T cells. LTB4 elicited BLT1-dependent chemotaxis in effector cells, but not in naive or central memory cells. Intravital microscopy showed that BLT1 signaling induced rapid integrin-mediated arrest of rolling effector and central memory cells in postcapillary venules. In competitive homing experiments, wild-type effector cells were three times more efficient at migrating to the inflamed peritoneal cavity than were BLT-deficient effector cells. These results identify LTB4-BLT1 as a potent nonchemokine pathway for cytotoxic effector cell traffic.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acute Disease
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Animals
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Chemotaxis, Leukocyte / immunology
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Flow Cytometry
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Immunologic Memory / immunology
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Integrins / immunology
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Leukotriene B4 / immunology*
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Muscle, Skeletal / blood supply
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Muscle, Skeletal / immunology
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Peritonitis / immunology
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Receptors, Leukotriene B4 / biosynthesis
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Receptors, Leukotriene B4 / immunology*
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Receptors, Lymphocyte Homing / immunology
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Signal Transduction / immunology*
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T-Lymphocyte Subsets / immunology*
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / metabolism
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T-Lymphocytes, Regulatory / immunology*
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T-Lymphocytes, Regulatory / metabolism
Substances
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Integrins
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Receptors, Leukotriene B4
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Receptors, Lymphocyte Homing
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Leukotriene B4