[Analysis of subtype of porcine endogenous retrovirus in two species of Chinese pigs]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):197-9.
[Article in Chinese]

Abstract

Objective: To analyze the subtype of porcine endogenous retrovirus (PERV) in two species of Chinese pigs-Wu zhishan pig and Banna minipig inbred; the total number of pigs being eighty six.

Methods: Three primers which are specific for the three PERV subtypes (subtypes A, B and C) were used to amplify the genome DNA extracted from the pigs.

Results: In all tested pigs' genome, subtype C was not found; in 77.9% pigs, double positive envAB were found; in 22.1% pigs, only A or B was found.

Conclusion: In the genome of peripheral leukocytes from Wuzhishan pig and Banna minipig inbred, only subtypes A and B exist, and envAB predominate.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Inbred Strains
  • DNA, Viral / genetics
  • Endogenous Retroviruses / classification*
  • Endogenous Retroviruses / genetics
  • Endogenous Retroviruses / isolation & purification
  • Species Specificity
  • Swine / virology*
  • Swine, Miniature
  • Zoonoses

Substances

  • DNA, Viral