Multicenter evaluation of the fully automated bactec MGIT 960 system and three molecular methods for the isolation and the identification of mycobacteria from clinical specimens

F Kontos; E Petinaki; S Nicolaou; Z Gitti; S Anagnostou; M Maniati; C Costopoulos; I Tselentis; A N Maniatis

doi:10.1016/s0732-8893(03)00078-6

Multicenter evaluation of the fully automated bactec MGIT 960 system and three molecular methods for the isolation and the identification of mycobacteria from clinical specimens

Diagn Microbiol Infect Dis. 2003 Aug;46(4):299-301. doi: 10.1016/s0732-8893(03)00078-6.

Authors

F Kontos¹, E Petinaki, S Nicolaou, Z Gitti, S Anagnostou, M Maniati, C Costopoulos, I Tselentis, A N Maniatis

Affiliation

¹ Department of Microbiology, Medical School, University Hospital of Larissa, Papakyriazi 22, Larissa, Greece.

PMID: 12944024
DOI: 10.1016/s0732-8893(03)00078-6

Abstract

A combination of Bactec MGIT 960 system, a PCR-based assay and a PCR-Restriction Analysis procedure (PRA) was assessed for the detection and identification of mycobacteria from clinical samples. The MGIT recovered 243 from 266 mycobacterial isolates. The PCR-based assay correctly identified all (234) Mycobacterium tuberculosis isolates, while 35 nontuberculous mycobacterial isolates were identified by PRA.

Publication types

Comparative Study
Evaluation Study
Multicenter Study

MeSH terms

Automation
Bacterial Typing Techniques / methods*
Colony Count, Microbial
Culture Media
DNA, Bacterial / analysis
Female
Humans
Male
Mycobacterium / classification*
Mycobacterium / isolation & purification
Mycobacterium Infections / diagnosis
Polymerase Chain Reaction*
Reagent Kits, Diagnostic
Sampling Studies
Sensitivity and Specificity

Substances

Culture Media
DNA, Bacterial
Reagent Kits, Diagnostic