Functional analysis of the actin-binding protein, tropomyosin 1, in neuroblastoma

M L Yager; J A I Hughes; F J Lovicu; P W Gunning; R P Weinberger; G M O'Neill

doi:10.1038/sj.bjc.6601201

Functional analysis of the actin-binding protein, tropomyosin 1, in neuroblastoma

Br J Cancer. 2003 Sep 1;89(5):860-3. doi: 10.1038/sj.bjc.6601201.

Authors

M L Yager¹, J A I Hughes, F J Lovicu, P W Gunning, R P Weinberger, G M O'Neill

Affiliation

¹ The Oncology Research Unit, The Children's Hospital at Westmead, Locked Bag 4001, Westmead 2145, NSW, Australia.

Abstract

Tropomyosin 1 (TM1) is downregulated in a number of transformed cell types, and exogenous expression of TM1 can restore actin organisation and reverse cellular transformation. We find that TM1 is also downregulated in human neuroblastoma cell lines, correlating with increasing malignancy. However, exogenous TM1 does not restore actin cytoskeleton organisation in neuroblastoma cells.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Actins / ultrastructure
Blotting, Western
Cell Transformation, Neoplastic / metabolism
Cytoskeleton / physiology*
Cytoskeleton / ultrastructure
Down-Regulation
Drosophila Proteins*
Fluorescent Antibody Technique
Humans
Neuroblastoma / metabolism*
Neurons / cytology
Neurons / metabolism
Proto-Oncogene Proteins c-myc / biosynthesis
Transfection
Tropomyosin / metabolism*
Tumor Cells, Cultured

Substances

Actins
Drosophila Proteins
Proto-Oncogene Proteins c-myc
TPM1 protein, human
Tm2 protein, Drosophila
Tropomyosin