The 9 kinds of Chinese traditional medicines which are used to cure diabetes including Xiaokewan, Yuquanwan, Kelening, Jiangtangshu, Jiangtang I-V are digested with HNO3-HClO4 (4:1) mixed acid. The 12 trace elements of copper, zinc, nickel, cobalt, manganese, chromium, molybdenum, iron, calcium, magnesium, cadmium and lead in the solution are determined by atomic absorption spectrometry. Its recovery ratio by standard addition is 97%-105%, and RSD is lower than 5%. This method has good accurate. The results obtained show that except Cd and Pb all other ten trace elements contents in the drug are high. The results of this paper provide useful data for studying the relation between the contents of these trace elements and the medical effect.