To investigate female gamete developmental competence and variability in cloned cattle, we performed ovum pick-up and in vitro fertilization in four sets of cloned heifers (n = 10, two sets of triplets and two sets of twins), and four groups of non-genetically related control animals (n = 13). A total of 304 OPU were performed and 1798 oocytes were recovered. Mean oocyte production per female per OPU (+/-S.D.) was similar for clone or control animals (5.7+/-2.9 versus 6.1+/-4.5, respectively), however, in two sets of clones variance for the number of oocytes recovered differed significantly (7.1 versus 23.9 and 7.3 versus 26.7, respectively P<0.001) between clone groups and their respective controls, cloned animals being more homogenous. After in vitro maturation, fertilization with semen from the same bull, and culture, the proportion of oocytes from cloned animals that developed into blastocysts was 35.0+/-29.2% and was not significantly different from controls (29.4+/-30.9). The CV for oocyte recovery, and blastocyst rates was lower in all groups of cloned animals than in controls. Nevertheless, within each set of clones, CV values indicated some degree of variability between animals, thus confirming that cloned cattle are not the exact phenotypic copy of each other. Despite the large number of oocytes analyzed, results should be interpreted with caution due to the limited number of cloned animals.