Four male sterile wheat with Aeglops kotschyi, Ae. variabilis, Ae. ventricosa and Ae. bicornis cytoplasm, were crossed with some wheat materials, and from the crosses a series of maintenance lines were selected. The APAGE(acid polyacrylamide gel electrophoresis) analysis indicated that most maintenance lines possessed the gliadin marker Gld1B3 of 1BL/1RS translocation chromosome. The root tip chromosomes of this maintenance lines had two satellite chromosomes, but other maintenance lines without Gld1B3 marker had the same four satellite chromosomes as other common wheat. Three male sterile wheats with Aeglops kotschyi, Ae. variabilis, Ae. ventricosa cytoplasm had the same fertility, but the male sterile wheat with Ae. bicornis cytoplasm could produce male sterile. Meantime, the paper discussed application of APAGE technology in breeding of non-1BL/1RS male sterile wheat.