Abstract
Objective:
To study the effect of lovastatin on the expression of IkappaBalpha and cell-cycle regulating proteins in MCF-7 cells.
Methods:
MCF-7 cells were treated with 4, 8 and 16 micro mol/L lovastatin for 48 - 72 h. The distribution of cell cycles was assayed by flow cytometry (FCM). The protein expression of IkappaBalpha, CDK4, p16, pRb in cytoplasm and IkappaBalpha in the nucleus were detected by Western blot.
Results:
Lovastatin could arrest cellcycle in the G(0)/G(1) phase in a dose- and time-dependent manner, obviously lowering the expression of IkappaBalpha, CDK4 and pRb protein level in the cytoplasm and increasing IkappaBalpha in the nucleus, but not on p16 protein level.
Conclusion:
Lovastatin can induce the arrest of cell cycle in G(0)/G(1) phase by affecting the expression of IkappaBalpha and cell-cycle regulating protein in MCF-7 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology*
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Breast Neoplasms / metabolism*
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Breast Neoplasms / pathology
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Cell Cycle / drug effects
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Cell Cycle Proteins / metabolism*
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Cell Line, Tumor
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase Inhibitor p16 / metabolism
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Cyclin-Dependent Kinases / metabolism
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Female
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Humans
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I-kappa B Proteins / metabolism*
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Lovastatin / pharmacology*
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NF-KappaB Inhibitor alpha
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NF-kappa B / antagonists & inhibitors
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Proto-Oncogene Proteins / metabolism
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Retinoblastoma Protein / metabolism
Substances
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Antineoplastic Agents
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor p16
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I-kappa B Proteins
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NF-kappa B
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NFKBIA protein, human
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Proto-Oncogene Proteins
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Retinoblastoma Protein
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NF-KappaB Inhibitor alpha
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Lovastatin
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CDK4 protein, human
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases