Biological hydrogen production is a process of applying anaerobic biological fermentation method to produce hydrogen from organic wastes. Hydrogen production potential of different microbiological source and organic wastes, as well as the composition of fermentation product in liquid phase, were studied in the batch culture experiments. Magnifying experiment of biohydrogen production was carried out in 30 L improvement UASB reactor using anaerobic fermentation of artificial wastewater containing starch by activated sludge process. The experiment confirm that hydrogen and carbon dioxide percentage of biogas were 40%-51% and 49%-60% under the condition of hydrogen production process (pH was 4.5-5.5 sludge VSS was 10 g/L; starch-COD was 5000 mg/L), and no methane was observed. Hydrogen purity was more than 97% by an absorption of sodium hydrate solution, continuous hydrogen production was more than 120 days.